文章发表前要求提供细胞鉴定的杂志（美德华生提供中英文细胞鉴定报告）

武汉大学病毒学国家重点实验室分享的研究数据，细胞污染率达46%。278个细胞样本中，128株细胞有污染情况，其中52株国内建立，76株来自国外；剩余150个细胞样本身份确认无误，但仅19株为国内建立。 （http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0170384#pone-0170384-t001）

 Nature和Science等愈来愈多的杂志要求作者提供论文中所用细胞的鉴定报告。 

Dr.Cell 实验室整理了一份目前明确要求提供细胞鉴定的杂志列表（包含但不仅限于以下期刊 ）： 

American Association for Cancer Research (AACR) Publications

• Cancer Discovery

• Cancer Epidemiology, Biomarkers and Prevention

• Cancer Immunology Research

• Cancer Prevention Research

• Cancer Research

• Clinical Cancer Research

• Molecular Cancer Research

• Molecular Cancer Therapeutics

Excerpt from Guidelines:  "If cell lines were used in the research, a statement addressing the following points must be included in the Materials and Methods section of the manuscript:

1. From where and when the cells were obtained          

2. Whether the cell lines have been tested and authenticated          

3. The method by which the cells were tested  
    How and when the cells were last tested"

 

Bentham Science Publications

• Anti-Cancer Agents in Medicinal Chemistry

• Current Biotechnology

• Current Cancer Drug Targets

• Current Cancer Therapy Reviews

• Current Medicinal Chemistry

• Current Molecular Medicine

• Current Molecular Pharmacology

• Current Pharmacogenomics and Personalized Medicine

• Current Radiopharmaceuticals

• Current Stem Cell Research & Therapy

• Current Traditional Medicine

• Current Topics in Medicinal Chemistry

• Letters in Drug Design & Discovery

• MicroRNA

• Mini-Reviews in Medicinal Chemistry

• Protein & Peptide Letters
       
       

Excerpt from Guidelines:  "Authors are therefore required to provide authentication of the origin and identity of the cells by performing cell profiling..."

 

BioMed Central Journals - includes but is not limited to:

• Biological Research

• BMC Biochemistry

• BMC Biotechnology

• BMC Cancer

• BMC Cell Biology

• BMC Dermatology

• BMC Developmental Biology

• BMC Gastroenterology

• BMC Genetics

• BMC Molecular Biology

• BMC Pharmacology and Toxicology

• BMC Women’s Health

• Breast Cancer Research

• Cancer & Metabolism

• Cancer Cell International

• Experimental Hematology & Oncology

• Gynecologic Oncology Research and Practice

• Journal of Experimental & Clinical Cancer Research

• Journal of Hematology & Oncology

• Journal of Ovarian Research

• Molecular Cancer

• Radiation Oncology

• Stem Cell Research & Therapy

• Thyroid Research
       
       

Excerpt from Guidelines:  “If human cell lines are used, authors are strongly encouraged to include the following information in their manuscript:

• the source of the cell line, including when and from where it was      obtained;

• whether the cell line has recently been authenticated and by what      method;    
  

• whether the cell line has recently been tested for mycoplasma      contamination.”

 

BioTechniques

Excerpt from Guidelines:  "Authors are encouraged to authenticate all cell lines used in their research efforts.  BioTechniques specifically requires authors to check any cell lines used in their experiments against the current database of misidentified cell lines curated by the International Cell Line Authentication Committee (ICLAC)..."

 

Cancer (Journal of the American Cancer Society)

Excerpt from Guidelines:  "Manuscripts based on research using cell lines must include a statement addressing the following points in the Methods section of the manuscript:

1. Where the cells were obtained from   
    Whether the cell lines have been tested and authenticated     

2.  The method by which the cells were tested"

 

Carcinogenesis

Excerpt from Guidelines:  "Carcinogenesis requires authors to report on the authentication of all cell lines reported in their research."

Cell Biochemistry and Biophysics

Excerpt from Guidelines: "Authentication of Cell Lines - Verification of the identity of cell lines must be included in Materials and Methods section of Research manuscripts and the methodology employed referenced in the Bibliography."

 

Cell Biology International

Excerpt from Guidelines: “Authors must ensure that any cell lines used (1) are certified as being the designated type, (2) have been checked to ensure they are free of contamination, and (3) have been used from young stock..."

Clinical Orthopaedics and Related Research

Excerpt from Guidelines: “Authors are encouraged to take a two-step approach to the problem. First, use existing resources to identify contaminated cell lines. ...Second, the cell line must be proven authentic, even if cross contamination has not been reported."

 

Endocrine Society Publications

• Endocrine Reviews     
   Endocrinology    

•  Molecular Endocrinology    

• Journal of Clinical Endocrinology & Metabolism

Excerpt from Guidelines:  "Endocrine Society editorial policy will require that all cell lines used and described in submitted manuscripts be authenticated."

In Vitro Cellular & Developmental Biology - Animal

Excerpt from Guidelines:  "Authors must state:

1. Whether you have tested and authenticated the cell lines utilized in      the research

2. List the method by which the cells were tested

3. List when the cells were last tested and where, or

4. State when the cells were obtained from a cell bank"

 

International Journal of Cancer

Excerpt from Guidelines:  "...studies dealing with established human (tumor) cell lines must provide authentication of the origin and identity of the cells. This is best achieved by DNA (STR) profiling..."

 

Investigative Ophthalmology and Visual Science (IOVS)

Excerpt from Guidelines:"In light of recent events, we strongly suggest that STR analysis be used to validate the authenticity of cell lines used in papers submitted to IOVS."

 

Journal of Hepatology
Excerpt from Guidelines:  "In the ‘Materials and methods’ section, the source of cells utilized (catalog number if obtained from vendor or cell bank, species, sex, strain, race, age of donor, whether primary or established) should be clearly indicated. The methods section should state if cell line authentication has been carried out and by what method (e.g., STR profiling) and when authentication testing was last performed for that cell line. Authors should be able to provide the test results upon request. Mycoplasma contamination testing status must also be reported."

 

Journal of Molecular Biology

Excerpt from Guidelines:  “In keeping with NIH guidelines, the Journal considers it to be good practice for cultured cell lines to be authenticated. A description of the methods used to authenticate cells should be included in the Materials and Methods section. Authors are expected to check that cell lines used in their experiments are free from mycoplasma infections.”

 

Journal of the National Cancer Institute

Excerpt from Guidelines:"Authors of provisionally accepted manuscripts that use cell lines should state the methods used to authenticate any cell lines used in their studies and should give the date of the last authentication."

 

Molecular Vision

Excerpt from Guidelines:"Manuscripts reporting experiments in which immortalized cell lines are used must include data and documentation that demonstrate that the actual cells used in the experiments reported in the manuscript exhibit the correct phenotype and genotype. Authors are must read the article written by the editors on the authentication of cell lines (The Editors. On authentication of cell lines. Mol. Vis. 2013; 19:1848-1851)."

 

Nature Publishing Group (NPG), includes but not limited to:

• Nature

• Nature Cell Biology

• Nature Methods

Excerpt from Guidelines:  Nature has a “Reporting Checklist for Life Sciences Articles” that includes the following under the topic of “Reagents”:   "7. Cell line identity:   a. Are any cell lines used in this paper listed in the database of commonly misidentified cell lines maintained by ICLAC (also available in NCBI Biosample)?   b. If yes, include in the Methods section a scientific justification of their use – indicate here on which page (or section and paragraph) the justification can be found.   c. For each cell line, include in the Methods section a statement that specifies:
- the source of the cell lines
- have the cell lines been authenticated? If so, by which method?
- have the cell lines been tested for mycoplasma contamination? In this checklist, indicate on which page (or section and paragraph) the information can be found."

 

Neuro-Oncology

Excerpt from Guidelines: "Methods and Materials:  If cell lines are used, include a statement that addresses:

• Where and when the cell line was obtained,

• Whether the cell lines have previously been tested and authenticated      (e.g., by a cell bank)

• The method by which the cell lines were tested, and

• How and when the cell lines were tested"

 

Society for Endocrinology Publications

• Endocrine-Related Cancer

• Journal of Endocrinology

• Journal of Molecular Endocrinology
       
       

Excerpt from Guidelines:  “We require that all cell lines are authenticated for correct origin."

 

PLOS ONE

Excerpt from Guidelines: "Authors should check established cell lines using the ICLAC Database of Cross-contaminated or Misidentified Cell Lines to confirm they are not misidentified or contaminated. Cell line authentication is recommended - e.g. by karyotyping, isozyme analysis, or short tandem repeats (STR) analysis - and may be required during peer review or after publication."

 

The Open Breast Cancer Journal (Bentham Open)

Excerpt from Guidelines: "Bentham OPEN recommends that all cell lines be authenticated prior to submitting a paper for review. ...Authentication is required when a new line is established or acquired, before freezing a cell line, if the performance of the line is not consistent or results are unexpected, if using more than one cell line, and before publication of the study."